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Image Search Results
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.
doi: 10.1007/s43630-021-00140-9
Figure Lengend Snippet: Fig. 9 Effect of flavonoids on UVA-affected HO-1 level and expression in NHDF. HO-1 protein level was analysed by Western blot (A) and HO-1 mRNA level by qPCR (B). Fibroblasts pre-treated with TA and QE (1 h) were non- irradiated (− UVA) or exposed to 7.5 J/cm2 (+ UVA). After incubation (24 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analy- sis are shown. *Significantly different from irradiated control cells at p = 0.05. #Significantly different from control cells at p = 0.05
Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722),
Techniques: Expressing, Western Blot, Irradiation, Incubation, Control
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.
doi: 10.1007/s43630-021-00140-9
Figure Lengend Snippet: Fig. 10 Effect of flavonoids on UVA-affected HO-1 level and expression in NHEK. HO-1 protein level was analysed by Western blot (A) and HO-1 mRNA level by qPCR (B). NHEK pre-treated with TA and QE (1 h) were non-irradiated (− UVA) or exposed to 10 J/ cm2 (+ UVA). After incubation (6 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analysis are shown. *Significantly different from irradiated control cells at p = 0.05. #Significantly different from control cells at p = 0.05
Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722),
Techniques: Expressing, Western Blot, Irradiation, Incubation, Control
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.
doi: 10.1007/s43630-021-00140-9
Figure Lengend Snippet: Fig. 11 Effect of flavonoids on UVA-affected NQO1 level and expression in NHDF. NQO1 protein level was analysed by Western blot (A) and NQO1 mRNA level by qPCR (B). Fibroblasts pre-treated with TA and QE (1 h) were non- irradiated (− UVA) or exposed to 7.5 J/cm2 (+ UVA). After incubation (24 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analy- sis are shown. *Significantly different from irradiated control cells at p = 0.05. #Significantly different from control cells at p = 0.05
Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722),
Techniques: Expressing, Western Blot, Irradiation, Incubation, Control
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Article Title: Effect of the flavonoids quercetin and taxifolin on UVA-induced damage to human primary skin keratinocytes and fibroblasts.
doi: 10.1007/s43630-021-00140-9
Figure Lengend Snippet: Fig. 12 Effect of flavonoids on UVA-affected NQO1 level and expression NHEK. NQO1 protein level was analysed by Western blot (A) and NQO1 mRNA level by qPCR (B). Keratinocytes pre-treated with TA and QE (1 h) were non- irradiated (− UVA) or exposed to 10 J/cm2 (+ UVA). After incubation (24 h), HO-1 level was evaluated as described in Materials and Methods. Data are normalized to the reference protein actin and gene GAPDH. Results are mean ± SD of 3 experiments employing cells from 3 donors. Representative examples of Western blot analy- sis are shown. *Significantly different from irradiated control cells at p = 0.05
Article Snippet: Western blotting luminol reagent (ImmunoCruzTM) for chemiluminescent horseradish peroxidase (HRP) detection, Nrf2 rabbit polyclonal IgG (C-20, sc-722),
Techniques: Expressing, Western Blot, Irradiation, Incubation, Control